Journal: BMC Pulmonary Medicine

Article Title: The effect of the mechanodynamic lung environment on fibroblast phenotype via the Flexcell

doi: 10.1186/s12890-024-03167-7

Figure Lengend Snippet: Summary of all Flexcell studies assessing the effects of the mechanical lungs on fibroblast phenotype and function

Article Snippet: MRC-5 lung fibroblasts 2D culture on BioFlex plates with LPS exposure , 5%, 10%, 15%, 20% , 0.1 Hz , 5% and 10% amplitudes increased MRC-5 proliferation, while 15% and 20% decreased proliferation rates , [ ] .

Techniques: Knock-Out, Comparison, Concentration Assay, Expressing, Phospho-proteomics, Control

Journal: Pharmaceutics

Article Title: Micellar Form of a Ferrocene-Containing Camphor Sulfonamide with Improved Aqueous Solubility and Tumor Curing Potential

doi: 10.3390/pharmaceutics15030791

Figure Lengend Snippet: Cell viability assay: cancerous (A549, H1299) and non-cancerous cells (MRC5) were treated with various concentrations of the free compound DK164, the micellar form of the compound (DK164-NP), and the corresponding amount of used copolymers (NP) for 72 h and the percentage of cell viability was calculated in comparison with control. Data are represented in mean ± SD.

Article Snippet: A549 and H1299 human lung carcinoma cell lines and MRC5 non-cancerous lung fibroblasts (from the ATTC collection of cell cultures) were purchased from LGC Limited, Augsburg, Germany.

Techniques: Viability Assay, Comparison, Control

Journal: Pharmaceutics

Article Title: Micellar Form of a Ferrocene-Containing Camphor Sulfonamide with Improved Aqueous Solubility and Tumor Curing Potential

doi: 10.3390/pharmaceutics15030791

Figure Lengend Snippet: Cell apoptosis: ( A ) Flow cytometry histograms of A549, H1299 and MRC5 cells, treated with the calculated IC50 concentrations of DK164-NP for 24 h or 48 h and then stained with Annexin V-FITC and Propidium Iodide (PI). The four quadrants in each panel correspond, respectively, to: necrotic cells (upper left); late apoptotic cells (upper right); early apoptotic cells (low right); viable cells (low left); ( B ) Quantitative analysis of the flow cytometry results from at least three independent experiments (n = 3) ±SD. Multiple comparisons function of two-way ANOVA (Tukey’s multiple comparison test) was used to compare the mean of treated samples with the mean of control column. Probability values were considered significant at the * p < 0.05, ** p < 0.01, **** p < 0.0001.

Article Snippet: A549 and H1299 human lung carcinoma cell lines and MRC5 non-cancerous lung fibroblasts (from the ATTC collection of cell cultures) were purchased from LGC Limited, Augsburg, Germany.

Techniques: Flow Cytometry, Staining, Comparison, Control

Journal: Pharmaceutics

Article Title: Micellar Form of a Ferrocene-Containing Camphor Sulfonamide with Improved Aqueous Solubility and Tumor Curing Potential

doi: 10.3390/pharmaceutics15030791

Figure Lengend Snippet: ( A ) Representative immunofluorescence images of the morphology of LC3-positive structures (green), vimentin staining (red), and DAPI-staining (blue) in A549, H1299, and MRC5 control cells and cells, treated with the calculated IC50 concentration of DK164-NP for 24 h; ( B ) Quantification of the LC3 positive cells and the LC3 average total area per cell from the images in A performed using ImageJ quantification tool (* p < 0.05). Quantification was performed from three experiments with >75 cells quantified for each condition. Error bars represent standard deviation (SD); AU: arbitrary units. Scale bars represent a distance of 15 µm.

Article Snippet: A549 and H1299 human lung carcinoma cell lines and MRC5 non-cancerous lung fibroblasts (from the ATTC collection of cell cultures) were purchased from LGC Limited, Augsburg, Germany.

Techniques: Immunofluorescence, Staining, Control, Concentration Assay, Standard Deviation

Journal: Pharmaceutics

Article Title: Micellar Form of a Ferrocene-Containing Camphor Sulfonamide with Improved Aqueous Solubility and Tumor Curing Potential

doi: 10.3390/pharmaceutics15030791

Figure Lengend Snippet: Activation and nuclear translocation of the p53 protein in A549 and MRC5 cells. ( A ) Representative immunofluorescence images of A549 and MRC5 control cells and cells treated with the calculated IC50 concentrations of DK164-NP for 48 h and labeled with anti-p53 antibody (red). DNA was co-stained with DAPI (blue); ( B ) Quantitative determination of the intensity of the nuclear p53-signal, normalized with nuclear DNA content, was performed by CellProfiler cell image analysis software (Broad Institute’s Imaging Platform, Cambridge, MA, USA) and GraphPad Prism 8.0 (Dotmatics, San Diego, CA, USA). A p -value < 0.05 was considered statistically significant, * p < 0.05. Quantification is based on three independent experiments with >50 cells scored for each condition. Error bars represent standard deviation (SD); AU: arbitrary unit. Scale bars represent a distance of 15 µm.

Article Snippet: A549 and H1299 human lung carcinoma cell lines and MRC5 non-cancerous lung fibroblasts (from the ATTC collection of cell cultures) were purchased from LGC Limited, Augsburg, Germany.

Techniques: Activation Assay, Translocation Assay, Immunofluorescence, Control, Labeling, Staining, Software, Imaging, Standard Deviation

Journal: Pharmaceutics

Article Title: Micellar Form of a Ferrocene-Containing Camphor Sulfonamide with Improved Aqueous Solubility and Tumor Curing Potential

doi: 10.3390/pharmaceutics15030791

Figure Lengend Snippet: Activation and nuclear translocation of the NFkB protein in lung cells. ( A ) Representative immunofluorescent images of A549, H1299, and MRC5 control cells and cells treated with the calculated IC50 concentrations of DK164-NP for 48 h and labeled with anti-NFkB antibody (green). DNA was co-stained with DAPI (blue); ( B ) Quantitative determination of the intensity of the nuclear NFkB-signal, normalized with nuclear DNA content, was performed by CellProfiler cell image analysis software (Broad Institute´s Imaging Platform, Cambridge, MA, USA) and GraphPad Prism v.8.0 (Dotmatics, San Diego, CA, USA). A p -value < 0.05 was considered statistically significant, ** p < 0.01. Quantification is based on three independent experiments with >50 cells scored for each condition. The error bars represent standard deviation (SD). AU: arbitrary unit. Scale bars represent a distance of 15 µm.

Article Snippet: A549 and H1299 human lung carcinoma cell lines and MRC5 non-cancerous lung fibroblasts (from the ATTC collection of cell cultures) were purchased from LGC Limited, Augsburg, Germany.

Techniques: Activation Assay, Translocation Assay, Control, Labeling, Staining, Software, Imaging, Standard Deviation